An Introduction to iNEXT.beta3D via Examples

The package iNEXT.beta3D (iNterpolation and EXTrapolation with beta diversity for three dimensions of biodiversity) is a sequel to iNEXT. The three dimensions (3D) of biodiversity include taxonomic diversity (TD), phylogenetic diversity (PD) and functional diversity (FD). This document provides an introduction demonstrating how to run iNEXT.beta3D. An online version iNEXT.beta3D Online is also available for users without an R background.

A unified framework based on Hill numbers (for TD) and their generalizations, Hill-Chao numbers (for PD and FD), is adopted in the package. TD quantifies the effective number of species, mean-PD (PD divided by tree depth) quantifies the effective number of lineages, and FD quantifies the effective number of virtual functional groups (or functional “species”). Thus, TD, mean-PD, and FD are all expressed in comparable units of species/lineage equivalents, allowing meaningful comparisons across the three dimensions of biodiversity; see Chao et al. (2021) for a review of the unified framework.

For each of the three dimensions, iNEXT.beta3D focuses on the multiplicative diversity decomposition (beta = gamma/alpha) for orders q = 0, 1 and 2 based on sampling data. Beta diversity quantifies the extent of among-assemblage differentiation, or the changes in species/lineages/functional-groups composition and abundance among assemblages. iNEXT.beta3D features standardized 3D estimates with a common sample size (for alpha and gamma diversity) or sample coverage (for alpha, beta and gamma diversity). iNEXT.beta3D also features coverage-based standardized estimates of four classes of dissimilarity measures.

Based on the rarefaction and extrapolation (R/E) method for Hill numbers (TD) of orders q = 0, 1 and 2, Chao et al. (2023b) developed the pertinent R/E theory for taxonomic beta diversity with applications to real-world spatial, temporal and spatio-temporal data. An application to Gentry’s global forest data along with a concise description of the theory is provided in Chao et al. (2023a). The extension to phylogenetic and functional beta diversity is generally parallel.

The iNEXT.beta3D package features two types of R/E sampling curves:

  1. Sample-size-based (or size-based) R/E sampling curves: This type of sampling curve plots standardized 3D gamma and alpha diversity with respect to sample size. Note that sample-size-based beta diversity is not generally a statistically valid measure (Chao et al. 2023b); therefore, the corresponding beta diversity sampling curve is not provided.

  2. Sample-coverage-based (or coverage-based) R/E sampling curves: This type of sampling curve plots standardized 3D gamma, alpha, and beta diversity as well as four classes of dissimilarity measures with respect to sample coverage (an objective measure of sample completeness).

Sufficient data are needed to run iNEXT.beta3D. If your data comprise only a few species and their abundances/phylogenies/traits, it is probable that the data lack sufficient information to run iNEXT.beta3D.

HOW TO CITE iNEXT.beta3D

If you publish your work based on results from iNEXT.beta3D, you should make reference to at least one of the following methodology papers (2023a, b) and also cite the iNEXT.beta3D package:

  • Chao, A., Chiu, C.-H., Hu, K.-H., and Zeleny, D. (2023a). Revisiting Alwyn H. Gentry’s forest transect data: a statistical sampling-model-based approach. Japanese Journal of Statistics and Data Science, 6, 861-884. (https://doi.org/10.1007/s42081-023-00214-1)

  • Chao, A., Thorn, S., Chiu, C.-H., Moyes, F., Hu, K.-H., Chazdon, R. L., Wu, J., Magnago, L. F. S., Dornelas, M., Zeleny, D., Colwell, R. K., and Magurran, A. E. (2023b). Rarefaction and extrapolation with beta diversity under a framework of Hill numbers: the iNEXT.beta3D standardization. Ecological Monographs e1588.(https://doi.org/10.1002/ecm.1588)

  • Chao, A., Lee, Y.-T., Lin, K.-L., and Chuang, P.-Y. (2026). The iNEXT.beta3D package: interpolation and extrapolation with beta diversity for three dimensions of biodiversity. R package version 1.1.0.

SOFTWARE NEEDED TO RUN iNEXT.beta3D IN R

HOW TO RUN iNEXT.beta3D:

The iNEXT.beta3D package is available from CRAN and can be downloaded from Anne Chao’s GitHub iNEXT.beta3D_github using the following commands. For a first-time installation, additional visualization extension package (ggplot2 from CRAN) and relevant package (iNEXT.3D from CRAN) must be installed and loaded.


## install iNEXT.beta3D package from CRAN
install.packages("iNEXT.beta3D")

## install the latest version from GitHub
install.packages('devtools')
library(devtools)
install_github('AnneChao/iNEXT.beta3D')

## import packages
library(iNEXT.beta3D)

There are three main functions in this package:

  • iNEXTbeta3D: computes standardized 3D estimates with a common sample size (for alpha and gamma diversity) or sample coverage (for alpha, beta and gamma diversity) for default sample sizes or coverage values. This function also computes coverage-based standardized 3D estimates of four classes of dissimilarity measures for default coverage values. In addition, this function also computes standardized 3D estimates with a particular vector of user-specified sample sizes or coverage values.

  • ggiNEXTbeta3D: Visualizes the output from the function iNEXTbeta3D.

  • DataInfobeta3D: Provides basic data information for (1) the reference sample in each assemblage, (2) the gamma reference sample in the pooled assemblage, and (3) the alpha reference sample in the joint assemblage.

DATA INPUT FORMAT

To assess beta diversity among assemblages, information on shared/unique species and their abundances is required. Thus, species identity (or any unique identification code) and assemblage affiliation must be provided in the data. In any input dataset, set row name of the data to be species name (or identification code) and column name to be assemblage name. Two types of species abundance/incidence data are supported:

  1. Individual-based abundance data (datatype = "abundance"): Input data for a single dataset with N assemblages consist of a species-by-assemblage abundance matrix/data.frame. Users can input several datasets which may represent data collected from various localities, regions, plots, time periods, …, etc. Input data for multiple datasets then consist of a list of matrices; each matrix represents a species-by-assemblage abundance matrix for one of the datasets. Different datasets can have different numbers of assemblages. iNEXTbeta3D computes beta diversity and dissimilarity among assemblages within each dataset.

  2. Sampling-unit-based incidence raw data (datatype = "incidence_raw"): Input data for a dataset with N assemblages consist of a list of matrices/data.frames, with each matrix representing a species-by-sampling-unit incidence raw matrix for one of the N assemblages; each element in the incidence raw matrix is 1 for a detection, and 0 for a non-detection. Users can input several datasets. Input data then consist of multiple lists with each list comprising a list of species-by-sampling-unit incidence matrices; see an example below. The number of sampling units can vary with datasets (but within a dataset, the number of sampling units in each assemblage must be the same). iNEXTbeta3D computes beta diversity and dissimilarity among assemblages within each dataset based on incidence-based frequency counts obtained from all sampling units.

Species abundance data format

We use the tree species abundance data collected from two rainforest fragments/localities in Brazil to assess beta diversity between Edge and Interior assemblages/habitats within each fragment; see Chao et al. (2023b) for analysis details. The data (named "Brazil_rainforests") consist of a list of two matrices (for two fragments named “Marim” and “Rebio2”, respectively); each matrix represents a species-by-assemblage abundance matrix, and there are two assemblages (“Edge” and “Interior”) in each fragment. The demo data are slightly different from those analyzed in Chao et al. (2023b) because seven species are removed from the original pooled data due to lack of phylogenetic information. Run the following code to view the data: (Here we only show the first 15 rows for each matrix.)


data(Brazil_rainforests)
Brazil_rainforests
#> $Marim
#>                            Edge Interior
#> Acosmium_lentiscifolium       1        0
#> Allophylus_petiolulatus       5        0
#> Alseis_involuta               2        0
#> Ampelocera_glabra             1        0
#> Andira_legalis                0        1
#> Andira_ormosioides            0        1
#> Apuleia_leiocarpa             1        0
#> Aspidosperma_illustre         0        3
#> Astrocaryum_aculeatissimum    1        0
#> Astronium_concinnum           4        1
#> Barnebydendron_riedelii       0        2
#> Bauhinia_forficata            1        0
#> Brosimum_glaucum              4        0
#> Calyptranthes_lucida          0        4
#> Campomanesia_lineatifolia     1        0
#> 
#> $Rebio2
#>                             Edge Interior
#> Albizia_polycephala            1        0
#> Allophylus_petiolulatus        3        3
#> Alseis_involuta                1        0
#> Amaioua_intermedia             0        1
#> Ampelocera_glabra              0        3
#> Anaxagorea_silvatica           0        6
#> Annona_dolabripetala           1        0
#> Aspidosperma_cylindrocarpon    2        0
#> Astrocaryum_aculeatissimum     7        1
#> Astronium_concinnum           12        1
#> Astronium_graveolens          13        1
#> Beilschmiedia_linharensis      1        0
#> Brosimum_glaucum               2        2
#> Brosimum_sp1                   0        1
#> Calyptranthes_lucida           2        1

Species incidence raw data format

We use tree species data collected from two second-growth rainforests, namely Cuatro Rios (CR) and Juan Enriquez (JE) in Costa Rica, as demo data to assess temporal beta diversity among three years (2005, 2011, and 2017) within each forest. Each year is designated as an assemblage. The data in each forest were collected from a 1-ha (50 m x 200 m) forest plot. Because individual trees of some species may exhibit intra-specific aggregation within a 1 ha area, they may not be suitable for modelling as independent sampling units. In this case, it is statistically preferable to first convert species abundance records in each forest to occurrence or incidence (detection/non-detection) data in subplots/quadrats; see Chao et al. (2023b) for analysis details.

Each 1-ha forest was divided into 100 subplots (each with 0.01 ha) and only species’ incidence records in each subplot were used to compute the incidence frequency for a species (i.e., the number of subplots in which that species occurred). By treating the incidence frequency of each species among subplots as a “proxy” for its abundance, the iNEXT.beta3D standardization can be adapted to deal with spatially aggregated data and to avoid the effect of intra-specific aggregation.

The data (named "Second_growth_forests") consist of two lists (for two forests named "CR_2005_2011_2017" and "JE_2005_2011_2017", respectively). Each list consists of three matrices; the first matrix represents the species-by-subplot incidence data in 2005, the second matrix represents the species-by-subplot incidence data in 2011, and the third matrix represents the species-by-subplot incidence data in 2017. Run the following code to view the incidence raw data: (Here we only show the first ten rows and six columns for each matrix; there are 100 columns/subplots in each forest and each year.)


data(Second_growth_forests)
Second_growth_forests
#> $CR_2005_2011_2017
#> $CR_2005_2011_2017$Year_2005
#>        Subplot_1 Subplot_2 Subplot_3 Subplot_4 Subplot_5 Subplot_6
#> Abaade         0         0         0         0         0         0
#> Alcflo         0         0         0         0         0         0
#> Alclat         0         1         0         0         0         0
#> Aliatl         0         0         0         0         0         0
#> Ampmac         0         0         0         0         0         0
#> Anacra         0         1         0         0         0         1
#> Annama         0         1         0         0         0         0
#> Annpap         0         0         0         0         0         0
#> Apemem         0         0         0         0         0         0
#> Ardfim         0         0         0         0         0         0
#> 
#> $CR_2005_2011_2017$Year_2011
#>        Subplot_1 Subplot_2 Subplot_3 Subplot_4 Subplot_5 Subplot_6
#> Abaade         0         0         0         0         0         0
#> Alcflo         0         0         0         0         0         0
#> Alclat         0         1         0         0         0         0
#> Aliatl         0         0         0         0         0         0
#> Ampmac         0         0         0         0         0         0
#> Anacra         0         1         0         0         0         1
#> Annama         0         0         0         0         0         0
#> Annpap         0         0         0         0         0         0
#> Apemem         0         0         0         0         0         0
#> Ardfim         0         0         0         0         0         0
#> 
#> $CR_2005_2011_2017$Year_2017
#>        Subplot_1 Subplot_2 Subplot_3 Subplot_4 Subplot_5 Subplot_6
#> Abaade         0         0         0         0         0         0
#> Alcflo         0         0         0         0         0         0
#> Alclat         0         1         0         0         0         0
#> Aliatl         0         0         0         0         0         0
#> Ampmac         0         0         0         0         0         0
#> Anacra         0         1         1         0         1         1
#> Annama         0         0         0         0         0         0
#> Annpap         0         0         0         0         0         0
#> Apemem         0         0         0         0         0         0
#> Ardfim         0         0         0         0         0         0
#> 
#> 
#> $JE_2005_2011_2017
#> $JE_2005_2011_2017$Year_2005
#>        Subplot_1 Subplot_2 Subplot_3 Subplot_4 Subplot_5 Subplot_6
#> Alccos         0         0         0         0         0         0
#> Alcflo         0         0         0         0         0         0
#> Alclat         0         0         0         0         0         0
#> Annpap         0         0         0         0         0         0
#> Apemem         0         0         0         0         0         0
#> Astcon         0         0         0         0         0         0
#> Bacgas         0         0         0         0         0         0
#> Brogui         0         0         0         0         0         0
#> Brolac         0         0         0         0         0         0
#> Byrcra         0         0         0         0         1         0
#> 
#> $JE_2005_2011_2017$Year_2011
#>        Subplot_1 Subplot_2 Subplot_3 Subplot_4 Subplot_5 Subplot_6
#> Alccos         0         0         0         0         0         0
#> Alcflo         0         0         0         0         0         0
#> Alclat         0         0         0         0         0         0
#> Annpap         0         0         0         0         0         0
#> Apemem         0         0         0         0         0         0
#> Astcon         0         0         0         0         0         0
#> Bacgas         0         0         0         0         0         0
#> Brogui         0         0         0         0         0         0
#> Brolac         0         0         0         0         0         0
#> Byrcra         0         0         0         0         1         0
#> 
#> $JE_2005_2011_2017$Year_2017
#>        Subplot_1 Subplot_2 Subplot_3 Subplot_4 Subplot_5 Subplot_6
#> Alccos         0         0         0         0         0         0
#> Alcflo         0         0         0         0         0         0
#> Alclat         0         0         0         0         0         0
#> Annpap         0         0         0         0         0         0
#> Apemem         0         0         0         0         0         0
#> Astcon         0         0         0         0         0         0
#> Bacgas         0         0         0         0         0         0
#> Brogui         0         0         0         0         0         0
#> Brolac         0         0         0         0         0         0
#> Byrcra         0         0         0         0         0         0

Phylogenetic tree format for PD

To perform PD analysis, the phylogenetic tree (in Newick format) spanned by species observed in all datasets must be stored in a data file. For example, the phylogenetic tree for all observed species (including species in both “Marim” and “Rebio2” fragments) is stored in a data file named "Brazil_tree" for demonstration purpose. A partial list of the tip labels and node labels are shown below.


data(Brazil_tree)
Brazil_tree
#> 
#> Phylogenetic tree with 185 tips and 117 internal nodes.
#> 
#> Tip labels:
#>   Carpotroche_brasiliensis, Casearia_ulmifolia, Casearia_sp2, Casearia_oblongifolia, Casearia_commersoniana, Rinorea_bahiensis, ...
#> Node labels:
#>   magnoliales_to_asterales, poales_to_asterales, , , , , ...
#> 
#> Rooted; includes branch length(s).

Species pairwise distance matrix format for FD

To perform FD analysis, the species-pairwise distance matrix (Gower distance computed from species traits) for species observed in all datasets must be stored in a matrix/data.frame format. Typically, the distance between any two species is computed from species traits using the Gower distance. In our demo data, the distance matrix for all species (including species in both “Marim” and “Rebio2” fragments) is stored in a data file named "Brazil_distM" for demonstration purpose. Here we only show the first three rows and three columns of the distance matrix.


data(Brazil_distM)
Brazil_distM
#>                          Carpotroche_brasiliensis Astronium_concinnum Astronium_graveolens
#> Carpotroche_brasiliensis                    0.000               0.522                0.522
#> Astronium_concinnum                         0.522               0.000                0.000
#> Astronium_graveolens                        0.522               0.000                0.000

MAIN FUNCTION: iNEXTbeta3D()

We first describe the main function iNEXTbeta3D() with default arguments:


iNEXTbeta3D(data, diversity = "TD", q = c(0, 1, 2), datatype = "abundance",
            base = "coverage", level = NULL, nboot = 10, conf = 0.95,
            PDtree = NULL, PDreftime = NULL, PDtype = 'AUC',  PDcut_number = 30,
            FDdistM = NULL, FDtype = "AUC", FDtau = NULL, FDcut_number = 30,
            by_pair = FALSE)

The arguments of this function are briefly described below, and will be explained in more detail through illustrative examples in later text. By default (with the standardization base = “coverage”), this function computes coverage-based standardized 3D gamma, alpha, beta diversity, and four dissimilarity indices for coverage up to one (for q = 1, 2) or up to the coverage of double the reference sample size (for q = 0). If users set the standardization base to base=“size”, this function computes size-based standardized 3D gamma and alpha diversity estimates up to double the reference sample size in each dataset. In addition, this function also computes standardized 3D estimates with a particular vector of user-specified sample sizes or coverage values.

Argument Description
data
  1. For datatype = “abundance”, species abundance data for a single dataset can be input as a matrix/data.frame (species-by-assemblage); data for multiple datasets can be input as a list of matrices/data.frames, with each matrix representing a species-by-assemblage abundance matrix for one of the datasets.
  2. For datatype = “incidence_raw”, data for a single dataset with N assemblages can be input as a list of matrices/data.frames, with each matrix representing a species-by-sampling-unit incidence matrix for one of the assemblages; data for multiple datasets can be input as multiple lists.
diversity selection of diversity type: diversity = “TD” = Taxonomic diversity, diversity = “PD” = Phylogenetic diversity, and diversity = “FD” = Functional diversity.
q a numerical vector specifying the diversity orders. Default is c(0, 1, 2).
datatype data type of input data: individual-based abundance data (datatype = “abundance”) or species-by-sampling-unit incidence matrix (datatype = “incidence_raw”) with all entries being 0 (non-detection) or 1 (detection).
base standardization base: coverage-based rarefaction and extrapolation for gamma, alpha, beta diversity, and four classes of dissimilarity indices (base = “coverage”), or size-based rarefaction and extrapolation for gamma and alpha diversity (base = “size”). Default is base = “coverage”.
level

A numerical vector specifying the particular values of sample coverage (between 0 and 1 when base = “coverage”) or sample sizes (base = “size”) that will be used to compute standardized diversity/dissimilarity. Asymptotic diversity estimator can be obtained by setting level = 1 (i.e., complete coverage for base = “coverage”).

By default (with base = “coverage”), this function computes coverage-based standardized 3D gamma, alpha, beta diversity, and four dissimilarity indices for coverage from 0.5 up to one (for q = 1, 2) or up to the coverage of double the reference sample size (for q = 0), in increments of 0.025. The extrapolation limit for beta diversity is defined as that for alpha diversity.

If users set base = “size”, this function computes size-based standardized 3D gamma and alpha diversity estimates based on 40 equally-spaced sample sizes/knots from sample size 1 up to double the reference sample size.
nboot a positive integer specifying the number of bootstrap replications when assessing sampling uncertainty and constructing confidence intervals. Bootstrap replications are generally time consuming. Set nboot = 0 to skip the bootstrap procedures. Default is nboot = 10. If more accurate results are required, set nboot = 100 (or nboot = 200).
conf a positive number < 1 specifying the level of confidence interval. Default is conf = 0.95.
PDtree (required argument for diversity = “PD”), a phylogenetic tree in Newick format spanned by all observed species across the input datasets.
PDreftime (argument only for diversity = “PD” and PDtype = “PD” or PDtype = “meanPD”), a numerical value specifying a fixed time reference point for phylogenetic diversity. Default is PDreftime = NULL, in which case the age of the root of the phylogenetic tree spanned by the pooled observed species is used.
PDtype (argument only for diversity = “PD”), select PD type: PDtype = “PD” for effective total branch length, PDtype = “meanPD” for effective number of equally divergent lineages, or PDtype = “AUC” for an overall measure that integrates mean phylogenetic diversity over all time reference points from zero to the age of the root. Default is PDtype = “AUC”.
PDcut_number (argument only for diversity = “PD” and PDtype = “AUC”), a positive integer greater than one specifying the number of equally spaced time reference points between zero and the age of the root used to numerically approximate the AUC. Default is PDcut_number = 30. A larger value can be used to obtain a more accurate AUC approximation.
FDdistM (required argument for diversity = “FD”), a species pairwise distance matrix for all species in the pooled assemblage.
FDtype (argument only for diversity = “FD”), select FD type: FDtype = “tau_value” for FD under a specified threshold value, or FDtype = “AUC” (area under the curve of tau-profile) for an overall FD which integrates all threshold values between zero and one. Default is FDtype = “AUC”.
FDtau (argument only for diversity = “FD” and FDtype=“tau_value”),
a numerical value between 0 and 1 specifying the tau value (threshold level) that will be used to compute FD. If FDtau = NULL (default), then the threshold level is set to be the mean distance between any two individuals randomly selected from the pooled dataset (i.e., quadratic entropy).
FDcut_number (argument only for diversity = “FD” and FDtype=“AUC”), a positive integer specifying the number of equally spaced sub-intervals used to partition the [0, 1] interval for numerical approximation of the AUC by integrating the tau-profile. Equivalently, the number of tau values that will be considered to compute the integrated AUC value. Default is FDcut_number = 30. A larger value can be used to obtain a more accurate AUC approximation.
by_pair a logical variable specifying whether diversity decomposition is performed for all assemblages as a whole or for every pair of assemblages. If by_pair = FALSE, alpha/beta/gamma diversity and dissimilarity are computed for all assemblages within each dataset as a whole; if by_pair = TRUE, the corresponding output is computed for every pair of assemblages. Default is FALSE.

This function returns an "iNEXTbeta3D" object which can be further used to make plots using the function ggiNEXTbeta3D() to be described below. Note that ggiNEXTbeta3D() only accepts output from iNEXTbeta3D() when by_pair = FALSE.

Output of the main function iNEXTbeta3D()

By default (with base = 'coverage'), the iNEXTbeta3D() function for each of the three dimensions (TD, PD, and FD) returns the "iNEXTbeta3D" object including seven data frames for each dataset:

  • gamma (standardized gamma diversity)
  • alpha (standardized alpha diversity)
  • beta (standardized beta diversity)
  • 1-C (standardized Sorensen-type non-overlap index)
  • 1-U (standardized Jaccard-type non-overlap index)
  • 1-V (standardized Sorensen-type turnover index)
  • 1-S (standardized Jaccard-type turnover index)

When users set base = 'size', the iNEXTbeta3D() function for each of the three dimensions (TD, PD, and FD) returns the "iNEXTbeta3D" object including two data frames for each dataset:

  • gamma (size-based standardized gamma diversity)
  • alpha (size-based standardized alpha diversity)

Size-based beta diversity and dissimilarity indices are not statistically valid measures and thus are not provided.

GRAPHIC DISPLAYS: FUNCTION ggiNEXTbeta3D()

The function ggiNEXTbeta3D() with default arguments is described as follows. This function only accepts output from iNEXTbeta3D when by_pair = FALSE.


ggiNEXTbeta3D(output, type = "B")  
Argument Description
output output from the function iNEXTbeta3D.
type

(argument only for base = "coverage"),

type = ‘B’ for plotting the rarefaction and extrapolation sampling curves for gamma, alpha, and beta diversity;

type = ‘D’ for plotting the rarefaction and extrapolation sampling curves for four dissimilarity indices.

Skip the argument for plotting size-based rarefaction and extrapolation sampling curves for gamma and alpha diversity.

The ggiNEXTbeta3D() function is a wrapper around the ggplot2 package to create an R/E curve using a single line of code. The resulting object is of class "ggplot", so it can be manipulated using the ggplot2 tools. Users can visualize the displays of coverage-based R/E sampling curves of gamma, alpha and beta diversity as well as four classes of dissimilarity indices by setting the parameter type.

TAXONOMIC DIVERSITY (TD): RAREFACTION/EXTRAPOLATION VIA EXAMPLES

EXAMPLE 1: Abundance data with default sample sizes or coverage values

First, we run the iNEXTbeta3D() function with Brazil_rainforests abundance data to compute coverage-based taxonomic gamma, alpha, beta diversity, and four dissimilarity indices under base = 'coverage' by running the following code:


## Coverage-based R/E Analysis with taxonomic diversity for abundance data
data(Brazil_rainforests)

output_TDc_abun = iNEXTbeta3D(data = Brazil_rainforests, diversity = 'TD', 
                              datatype = 'abundance', base = "coverage", nboot = 10)
output_TDc_abun

The output contains seven data frames: gamma, alpha, beta, 1-C, 1-U, 1-V, 1-S. Each data frame includes the dataset name (Dataset), combinations of assemblage pairs (Pair, if the calculation is not performed by pairs, this column is absent), the diversity order of q (Order.q), the target standardized coverage value (SC), the corresponding sample size (Size), the estimated diversity/dissimilarity value (Alpha/Beta/Gamma/Dissimilarity), Method (Rarefaction, Observed, or Extrapolation, depending on whether the target coverage is less than, equal to, or greater than the coverage of the reference sample), standard error of standardized estimate (s.e.), the bootstrap lower and upper confidence limits for the diversity/dissimilarity with a default significance level of 0.95 (LCL, UCL). These estimates and confidence intervals are then used to plot the rarefaction and extrapolation curves.

Our diversity/dissimilarity estimates and related statistics in the default output are displayed for the standardized coverage value from 0.5 to the coverage value of twice the reference sample size (for q = 0), or from 0.5 to 1.0 (for q = 1 and 2), in increments of 0.025. In addition, the results for the following four coverage value are also added: SC(n, alpha), SC(2n, alpha), SC(n, gamma) and SC(2n, gamma) if these values are in the above-specified range. Here SC(n, alpha) and SC(2n, alpha) represent, respectively, the coverage estimate for the alpha reference sample size n and the extrapolated sample with size 2n in the joint assemblage. These values can be found as SC(n) and SC(2n) for "Joint assemblage (for alpha)" in the column “Assemblage” from the output of the function DataInfobeta3D; see later text. Similar definitions pertain to SC(n, gamma) and SC(2n, gamma) for the gamma reference sample; these two values can also be found as SC(n) and SC(2n) for "Pooled assemblage (for gamma)" in the column “Assemblage” from the output of the function DataInfobeta3D. For beta diversity and dissimilarity, the observed sample coverage and extrapolation limit are defined the same as the alpha diversity. The corresponding coverage values for incidence data are denoted as, respectively, SC(T, alpha), SC(2T, alpha), SC(T, gamma) and SC(2T, gamma) in the output.

Because all the diversity/dissimilarity estimates are computed for the standardized coverage range values starting from 0.5, the default setting with level = NULL does not work if the observed sample coverage in the alpha/gamma reference sample is less than 50%. In this case, readers should specify sample coverage values using the argument level, instead of using level = NULL. The suggested maximum coverage value that readers can specify is SC(2n, alpha). Beyond the limit, beta diversity and dissimilarity estimates may be subject to some bias. Below we show the output for taxonomic beta diversity between the “Edge” and “Interior” habitats in the “Marim” fragment.

#>    Dataset Order.q    SC Size Beta                Method  s.e.   LCL  UCL
#> 1    Marim       0 0.500  148 1.11           Rarefaction 0.083 0.947 1.27
#> 2    Marim       0 0.525  162 1.11           Rarefaction 0.084 0.944 1.27
#> 3    Marim       0 0.550  178 1.10           Rarefaction 0.084 0.941 1.27
#> 4    Marim       0 0.575  195 1.10           Rarefaction 0.084 0.937 1.27
#> 5    Marim       0 0.600  213 1.10           Rarefaction 0.085 0.932 1.26
#> 6    Marim       0 0.625  233 1.09           Rarefaction 0.086 0.927 1.26
#> 7    Marim       0 0.650  255 1.09           Rarefaction 0.087 0.921 1.26
#> 8    Marim       0 0.675  279 1.09           Rarefaction 0.088 0.916 1.26
#> 9    Marim       0 0.696  302 1.09 Observed_SC(n, alpha) 0.089 0.911 1.26
#> 10   Marim       0 0.700  306 1.09         Extrapolation 0.090 0.911 1.26
#> 11   Marim       0 0.725  336 1.08         Extrapolation 0.090 0.909 1.26
#> 12   Marim       0 0.750  368 1.08         Extrapolation 0.089 0.911 1.26
#> 13   Marim       0 0.775  403 1.08         Extrapolation 0.087 0.915 1.26
#> 14   Marim       0 0.800  443 1.09         Extrapolation 0.085 0.920 1.25
#> 15   Marim       0 0.825  488 1.09         Extrapolation 0.084 0.926 1.25
#> 16   Marim       0 0.850  541 1.09         Extrapolation 0.085 0.926 1.26
#> 17   Marim       0 0.855  552 1.09 Observed_SC(n, gamma) 0.086 0.924 1.26
#> 18   Marim       0 0.875  602 1.09         Extrapolation 0.094 0.911 1.28
#> 19   Marim       0 0.876  604 1.09  Extrap_SC(2n, alpha) 0.094 0.910 1.28
#> 20   Marim       1 0.500  148 1.11           Rarefaction 0.079 0.956 1.26
#> 21   Marim       1 0.525  162 1.11           Rarefaction 0.079 0.954 1.26
#> 22   Marim       1 0.550  178 1.11           Rarefaction 0.078 0.952 1.26
#> 23   Marim       1 0.575  195 1.10           Rarefaction 0.079 0.949 1.26
#> 24   Marim       1 0.600  213 1.10           Rarefaction 0.079 0.947 1.26
#> 25   Marim       1 0.625  233 1.10           Rarefaction 0.079 0.944 1.25
#> 26   Marim       1 0.650  255 1.10           Rarefaction 0.080 0.941 1.25
#> 27   Marim       1 0.675  279 1.09           Rarefaction 0.080 0.938 1.25
#> 28   Marim       1 0.696  302 1.09 Observed_SC(n, alpha) 0.080 0.937 1.25
#> 29   Marim       1 0.700  306 1.09         Extrapolation 0.080 0.937 1.25
#> 30   Marim       1 0.725  336 1.09         Extrapolation 0.080 0.936 1.25
#> 31   Marim       1 0.750  368 1.09         Extrapolation 0.079 0.935 1.25
#> 32   Marim       1 0.775  403 1.09         Extrapolation 0.078 0.934 1.24
#> 33   Marim       1 0.800  443 1.08         Extrapolation 0.077 0.933 1.24
#> 34   Marim       1 0.825  488 1.08         Extrapolation 0.076 0.931 1.23
#> 35   Marim       1 0.850  541 1.07         Extrapolation 0.075 0.927 1.22
#> 36   Marim       1 0.855  552 1.07 Observed_SC(n, gamma) 0.076 0.925 1.22
#> 37   Marim       1 0.875  602 1.07         Extrapolation 0.077 0.917 1.22
#> 38   Marim       1 0.876  604 1.07  Extrap_SC(2n, alpha) 0.078 0.917 1.22
#> 39   Marim       1 0.900  678 1.06         Extrapolation 0.081 0.906 1.22
#> 40   Marim       1 0.925  775 1.06         Extrapolation 0.084 0.898 1.23
#> 41   Marim       1 0.950  912 1.06         Extrapolation 0.086 0.894 1.23
#> 42   Marim       1 0.969 1075 1.07  Extrap_SC(2n, gamma) 0.086 0.898 1.23
#> 43   Marim       1 0.975 1147 1.07         Extrapolation 0.085 0.900 1.24
#> 44   Marim       1 1.000  Inf 1.10         Extrapolation 0.074 0.958 1.25
#> 45   Marim       2 0.500  148 1.10           Rarefaction 0.069 0.967 1.24
#> 46   Marim       2 0.525  162 1.10           Rarefaction 0.068 0.965 1.23
#> 47   Marim       2 0.550  178 1.10           Rarefaction 0.067 0.964 1.23
#> 48   Marim       2 0.575  195 1.09           Rarefaction 0.067 0.963 1.23
#> 49   Marim       2 0.600  213 1.09           Rarefaction 0.067 0.961 1.22
#> 50   Marim       2 0.625  233 1.09           Rarefaction 0.066 0.959 1.22
#> 51   Marim       2 0.650  255 1.09           Rarefaction 0.066 0.958 1.22
#> 52   Marim       2 0.675  279 1.09           Rarefaction 0.066 0.957 1.22
#> 53   Marim       2 0.696  302 1.08 Observed_SC(n, alpha) 0.066 0.956 1.21
#> 54   Marim       2 0.700  306 1.08         Extrapolation 0.066 0.956 1.21
#> 55   Marim       2 0.725  336 1.08         Extrapolation 0.065 0.958 1.21
#> 56   Marim       2 0.750  368 1.08         Extrapolation 0.063 0.961 1.21
#> 57   Marim       2 0.775  403 1.09         Extrapolation 0.061 0.966 1.21
#> 58   Marim       2 0.800  443 1.09         Extrapolation 0.059 0.972 1.20
#> 59   Marim       2 0.825  488 1.09         Extrapolation 0.056 0.979 1.20
#> 60   Marim       2 0.850  541 1.09         Extrapolation 0.053 0.986 1.20
#> 61   Marim       2 0.855  552 1.09 Observed_SC(n, gamma) 0.053 0.987 1.20
#> 62   Marim       2 0.875  602 1.09         Extrapolation 0.052 0.989 1.19
#> 63   Marim       2 0.876  604 1.09  Extrap_SC(2n, alpha) 0.052 0.990 1.19
#> 64   Marim       2 0.900  678 1.09         Extrapolation 0.052 0.991 1.20
#> 65   Marim       2 0.925  775 1.09         Extrapolation 0.052 0.991 1.20
#> 66   Marim       2 0.950  912 1.09         Extrapolation 0.052 0.991 1.20
#> 67   Marim       2 0.969 1075 1.09  Extrap_SC(2n, gamma) 0.053 0.990 1.20
#> 68   Marim       2 0.975 1147 1.09         Extrapolation 0.053 0.989 1.20
#> 69   Marim       2 1.000  Inf 1.09         Extrapolation 0.066 0.960 1.22

Run the following code to display the two types of curves:


## Coverage-based R/E curves for taxonomic gamma, alpha and beta diversity 
ggiNEXTbeta3D(output_TDc_abun, type = 'B')


## Coverage-based R/E curves for four taxonomic dissimilarity indices
ggiNEXTbeta3D(output_TDc_abun, type = 'D')

The following commands return the size-based R/E sampling curves for gamma and alpha taxonomic diversity:


## Size-based R/E curves with taxonomic gamma and alpha diversity
data(Brazil_rainforests)
output_TDs_abun = iNEXTbeta3D(data = Brazil_rainforests, diversity = 'TD', 
                              datatype = 'abundance', base = "size", nboot = 10)

ggiNEXTbeta3D(output_TDs_abun)

EXAMPLE 2: Incidence data with default sample sizes or coverage values (all assemblages as a whole)

We can also use incidence raw data (Second_growth_forests) to compute coverage-based standardized gamma, alpha, beta diversity, and four dissimilarities under base = 'coverage', and also size-based standardized gamma and alpha diversity. Run the following code to perform incidence data analysis. The output data frame is similar to that based on abundance data and thus is omitted.


## Coverage-based R/E Analysis with taxonomic diversity for incidence raw data (all assemblages as a whole)
data(Second_growth_forests)

output_TDc_inci = iNEXTbeta3D(data = Second_growth_forests, diversity = 'TD', 
                              datatype = 'incidence_raw', base = "coverage", nboot = 10)
output_TDc_inci

The same procedures can be applied to incidence data. Based on the demo dataset, we display below the coverage-based R/E curves for temporal beta diversity among the three years (2005, 2011, and 2017) in the two second-growth forests (CR and JE) by running the following code:


## Coverage-based R/E curves with taxonomic gamma, alpha and beta diversity 

ggiNEXTbeta3D(output_TDc_inci, type = 'B')

The following commands return the size-based R/E sampling curves for gamma and alpha taxonomic diversity:


## Size-based R/E curves with taxonomic gamma and alpha diversity (all assemblages as a whole)
data(Second_growth_forests)

output_TDs_inci = iNEXTbeta3D(data = Second_growth_forests, diversity = 'TD', 
                              datatype = 'incidence_raw', base = "size", nboot = 10)

ggiNEXTbeta3D(output_TDs_inci)

EXAMPLE 3: Incidence data for every pair of assemblages with user-specified sample sizes or coverage values

As with abundance data, user can also specify sample sizes (i.e. number of sampling units) or coverage values to obtain the pertinent output for every pair of assemblages. The code for examples is given below with two user-specified levels of sample coverage values (e.g., 90% and 95%), but the output is omitted.


## Coverage-based R/E Analysis for every pair of assemblages with taxonomic diversity for incidence data
data(Second_growth_forests)

output_TDc_inci_byuser = iNEXTbeta3D(data = Second_growth_forests, diversity = 'TD', 
                                     datatype = 'incidence_raw', base = "coverage", 
                                     nboot = 10, level = c(0.9, 0.95), by_pair = TRUE)
output_TDc_inci_byuser

The following commands return the TD estimates for every pair of assemblages with two user-specified levels of sample sizes (e.g., 100 and 200).


## Size-based R/E for every pair of assemblages with taxonomic gamma and alpha diversity
data(Second_growth_forests)

output_TDs_inci_byuser = iNEXTbeta3D(data = Second_growth_forests, diversity = 'TD', 
                                     datatype = 'incidence_raw', base = "size", 
                                     nboot = 10, level = c(100, 200), by_pair = TRUE)
output_TDs_inci_byuser

PHYLOGENETIC DIVERSITY (PD): RAREFACTION/EXTRAPOLATION VIA EXAMPLES

EXAMPLE 4: Abundance data with default sample sizes or coverage values

As with taxonomic diversity, iNEXT.beta3D computes coverage-based standardized phylogenetic gamma, alpha, beta diversity as well as four classes of phylogenetic dissimilarity indices; it also computes size-based standardized phylogenetic gamma and alpha diversity. The species names (or identification codes) in the phylogenetic tree must exactly match with those in the corresponding species abundance/incidence data. Two types of phylogenetic rarefaction and extrapolation curves (coverage- and size-based sampling curves) are also provided.

The required argument for performing PD analysis is PDtree. For example, the phylogenetic tree for all observed species (including species in both “Marim” and “Rebio2” fragments) is stored in a data file named "Brazil_tree". Then we enter the argument PDtree = Brazil_tree. Three optional arguments are PDtype, PDreftime, and PDcut_number. There are three options for PDtype: "PD" (effective total branch length), "meanPD" (effective number of equally divergent lineages, meanPD = PD/tree depth), or "AUC" (an overall measure that integrates mean phylogenetic diversity over all time reference points from zero to the age of the root). Default is PDtype = "AUC".

For PDtype = "PD" and PDtype = "meanPD", PDreftime specifies a fixed time reference point for phylogenetic diversity. If PDreftime = NULL, the age of the root of the phylogenetic tree spanned by the pooled observed species is used.

For PDtype = "AUC", mean phylogenetic diversity is integrated over all time reference points from zero to the age of the root. PDcut_number specifies the number of equally spaced time reference points used to numerically approximate the AUC.

Default is PDcut_number = 30. A larger value can be used to obtain a more accurate AUC approximation. Run the following code to perform PD analysis. The output data frame is similar to that based on abundance data and thus is omitted.


## Coverage-based R/E Analysis with phylogenetic diversity for abundance data
data(Brazil_rainforests)
data(Brazil_tree)

output_PDc_abun = iNEXTbeta3D(data = Brazil_rainforests, diversity = 'PD', 
                              datatype = 'abundance', base = "coverage", nboot = 10, 
                              PDtree = Brazil_tree, PDtype = 'AUC', PDcut_number = 30)
output_PDc_abun

Run the following code to display the R/E curves for phylogenetic gamma, alpha, and beta diversity:


## Coverage-based R/E sampling curves for phylogenetic gamma, alpha and beta diversity
ggiNEXTbeta3D(output_PDc_abun, type = 'B')

The following commands return the size-based R/E sampling curves for gamma and alpha phylogenetic diversity:


## Size-based R/E curves for phylogenetic gamma and alpha diversity
data(Brazil_rainforests)
data(Brazil_tree)

output_PDs_abun = iNEXTbeta3D(data = Brazil_rainforests, diversity = 'PD', 
                              datatype = 'abundance', base = "size", nboot = 10, 
                              PDtree = Brazil_tree, PDtype = 'AUC', PDcut_number = 30)
ggiNEXTbeta3D(output_PDs_abun)

FUNCTIONAL DIVERSITY (FD): RAREFACTION/EXTRAPOLATION VIA EXAMPLES

EXAMPLE 5: Abundance data with default sample sizes or coverage values

As with taxonomic and phylogenetic diversity, iNEXT.beta3D computes coverage-based standardized functional gamma, alpha, beta diversity as well as four classes of functional dissimilarity indices; it also computes size-based standardized functional gamma and alpha diversity. The species names (or identification codes) in the distance matrix must exactly match with those in the corresponding species abundance/incidence data. Two types of functional rarefaction and extrapolation curves (coverage- and size-based sampling curves) are also provided.

The required argument for performing FD analysis is FDdistM. For example, the distance matrix for all species (including species in both “Marim” and “Rebio2” fragments) is stored in a data file named "Brazil_distM". Then we enter the argument FDdistM = Brazil_distM. Three optional arguments are (1) FDtype: FDtype = "AUC" means FD is computed from the area under the curve of a tau-profile by integrating all plausible threshold values between zero and one; FDtype = "tau_value" means FD is computed under a specific threshold value to be specified in the argument FDtau. (2) FDtau: a numerical value specifying the tau value (threshold level) that will be used to compute FD. If FDtype = "tau_value" and FDtau = NULL, then the threshold level is set to be the mean distance between any two individuals randomly selected from the pooled data over all datasets (i.e., quadratic entropy). (3) FDcut_number is a positive integer specifying the number of equally spaced sub-intervals used to partition the [0, 1] interval for numerical approximation of the AUC. Default is FDcut_number = 30. If more accurate integration is desired, then use a larger integer. Run the following code to perform FD analysis. The output data frame is similar to that based on abundance data and thus is omitted; see later graphical display of the output.


## Coverage-based R/E Analysis with functional diversity for abundance data - FDtype = 'AUC' (area 
## under curve) by considering all threshold values between zero and one
data(Brazil_rainforests)
data(Brazil_distM)

output_FDc_abun = iNEXTbeta3D(data = Brazil_rainforests, diversity = 'FD', 
                              datatype = 'abundance', base = "coverage", nboot = 10, 
                              FDdistM = Brazil_distM, FDtype = 'AUC', FDcut_number = 30)
output_FDc_abun

Run the following code to display the R/E curves for functional gamma, alpha, and beta diversity:


## Coverage-based R/E sampling curves for functional gamma, alpha and beta diversity
ggiNEXTbeta3D(output_FDc_abun, type = 'B')

The following commands return the size-based R/E sampling curves for gamma and alpha functional diversity:


## Size-based R/E curves for functional gamma and alpha diversity
data(Brazil_rainforests)
data(Brazil_distM)

output_FDs_abun = iNEXTbeta3D(data = Brazil_rainforests, diversity = 'FD', 
                              datatype = 'abundance', base = "size", nboot = 10, 
                              FDdistM = Brazil_distM, FDtype = 'AUC', FDcut_number = 30)
ggiNEXTbeta3D(output_FDs_abun)

DATA INFORMATION: FUNCTION DataInfobeta3D()

The function DataInfobeta3D() provides basic data information for (1) the reference sample in each individual assemblage, (2) the gamma reference sample in the pooled assemblage, and (3) the alpha reference sample in the joint assemblage. The function DataInfobeta3D() with default arguments is shown below:

DataInfobeta3D(data, diversity = "TD", datatype = "abundance",
               PDtree = NULL, PDreftime = NULL, PDtype = "meanPD",
               FDdistM = NULL, FDtype = "AUC", FDtau = NULL, by_pair = FALSE)  

Arguments shared by DataInfobeta3D() and iNEXTbeta3D() have the same definitions as described above. Running the DataInfobeta3D() function returns basic data information including sample size, observed species richness, two sample coverage estimates (SC(n) and SC(2n)) as well as other relevant information in each of the three dimensions of diversity. We use Brazil_rainforests data to demonstrate the function for each dimension.

## Data information for taxonomic diversity
data(Brazil_rainforests)
DataInfobeta3D(data = Brazil_rainforests, diversity = 'TD', datatype = 'abundance')
#>   Dataset        Assemblage   n S.obs SC(n) SC(2n) f1 f2 f3 f4 f5
#> 1   Marim              Edge 158    84 0.691  0.852 49 18  8  4  1
#> 2   Marim          Interior 144    80 0.704  0.899 43 23  7  5  0
#> 3   Marim Pooled assemblage 302   119 0.855  0.969 44 34 17  9  7
#> 4   Marim  Joint assemblage 302   164 0.696  0.876 92 41 15  9  1
#> 5  Rebio2              Edge 162    70 0.754  0.895 40 17  4  2  0
#> 6  Rebio2          Interior 168    74 0.763  0.877 40 13  8  4  4
#> 7  Rebio2 Pooled assemblage 330   118 0.819  0.901 60 18 15  5  3
#> 8  Rebio2  Joint assemblage 330   144 0.758  0.886 80 30 12  6  4

Output description:

  • Dataset = the input datasets.

  • Pair = combinations of assemblage pairs; this column is included only when the analysis is performed for every pair of assemblages.

  • Assemblage = Individual assemblages, 'Pooled assemblage' (for gamma) or 'Joint assemblage' (for alpha).

  • n = number of observed individuals in the reference sample (sample size).

  • S.obs = number of observed species in the reference sample.

  • SC(n) = sample coverage estimate of the reference sample.

  • SC(2n) = sample coverage estimate of twice the reference sample size.

  • f1-f5 = the first five species abundance frequency counts in the reference sample.


## Data information for mean phylogenetic diversity
data(Brazil_rainforests)
data(Brazil_tree)
DataInfobeta3D(data = Brazil_rainforests, diversity = 'PD', datatype = 'abundance', 
               PDtype = 'meanPD', PDtree = Brazil_tree, PDreftime = NULL)
#>   Dataset        Assemblage   n S.obs SC(n) SC(2n) PD.obs f1* f2*   g1   g2 Reftime
#> 1   Marim              Edge 158    84 0.691  0.852   8805  49  26 3278 2188     400
#> 2   Marim          Interior 144    80 0.704  0.899   8436  43  28 2974 1935     400
#> 3   Marim Pooled assemblage 302   119 0.855  0.969  11842  44  39 3172 2995     400
#> 4   Marim  Joint assemblage 302   164 0.696  0.876  17241  92  54 6252 4123     400
#> 5  Rebio2              Edge 162    70 0.754  0.895   7874  40  23 3648 1717     400
#> 6  Rebio2          Interior 168    74 0.763  0.877   8360  40  17 3365 1954     400
#> 7  Rebio2 Pooled assemblage 330   118 0.819  0.901  11979  60  23 5063 1637     400
#> 8  Rebio2  Joint assemblage 330   144 0.758  0.886  16234  80  40 7013 3671     400

For both PDtype = "PD" and PDtype = "meanPD", the output information is described as follows:

  • Dataset, Pair, Assemblage, n, S.obs, SC(n) and SC(2n): definitions are the same as in the TD output.

  • PD.obs = the observed total branch length in the phylogenetic tree spanned by all observed species.

  • f1*,f2* = the number of singletons and doubletons in the node/branch abundance set.

  • g1,g2 = the total branch length of those singletons/doubletons in the node/branch abundance set.

  • Reftime = the fixed time reference point for phylogenetic diversity. If PDreftime = NULL, the age of the root of the phylogenetic tree spanned by the pooled observed species is used.


## Data information for phylogenetic diversity when all time reference points are considered
data(Brazil_rainforests)
data(Brazil_tree)

DataInfobeta3D(data = Brazil_rainforests, diversity = 'PD',
               datatype = 'abundance', PDtype = 'AUC',
               PDtree = Brazil_tree)
               
#>   Dataset        Assemblage   n S.obs SC(n) SC(2n) Tree.depth.min Tree.depth.max
#> 1   Marim              Edge 158    84 0.691  0.852              0            400
#> 2   Marim          Interior 144    80 0.704  0.899              0            400
#> 3   Marim Pooled assemblage 302   119 0.855  0.969              0            400
#> 4   Marim  Joint assemblage 302   164 0.696  0.876              0            400
#> 5  Rebio2              Edge 162    70 0.754  0.895              0            400
#> 6  Rebio2          Interior 168    74 0.763  0.877              0            400
#> 7  Rebio2 Pooled assemblage 330   118 0.819  0.901              0            400
#> 8  Rebio2  Joint assemblage 330   144 0.758  0.886              0            400

For PDtype = "AUC" the output information is described as follows:

  • Dataset, Pair, Assemblage, n, S.obs, SC(n) and SC(2n): definitions are the same as in the TD output.

  • Tree.depth.min = the minimum time reference point used for calculating the integrated mean phylogenetic diversity; this value is zero.

  • Tree.depth.max = the maximum time reference point used for calculating the integrated mean phylogenetic diversity; this value is the age of the root of the phylogenetic tree spanned by the pooled observed species.


## Data information for functional diversity (under a specified threshold level, FDtype = 'tau_value')
data(Brazil_rainforests)
data(Brazil_distM)
DataInfobeta3D(data = Brazil_rainforests, diversity = 'FD', datatype = 'abundance', 
               FDdistM = Brazil_distM, FDtype = 'tau_value', FDtau = NULL)
#>   Dataset        Assemblage   n S.obs SC(n) SC(2n) a1* a2* h1 h2   Tau
#> 1   Marim              Edge 158    84 0.691  0.852   0   0  0  0 0.343
#> 2   Marim          Interior 144    80 0.704  0.899   0   0  0  0 0.343
#> 3   Marim Pooled assemblage 302   119 0.855  0.969   0   0  0  0 0.343
#> 4   Marim  Joint assemblage 302   164 0.696  0.876   0   0  0  0 0.343
#> 5  Rebio2              Edge 162    70 0.754  0.895   0   0  0  0 0.343
#> 6  Rebio2          Interior 168    74 0.763  0.877   0   0  0  0 0.343
#> 7  Rebio2 Pooled assemblage 330   118 0.819  0.901   0   0  0  0 0.343
#> 8  Rebio2  Joint assemblage 330   144 0.758  0.886   0   0  0  0 0.343

For FDtype = "tau_value" the output information is described as follows:

  • Dataset, Pair, Assemblage, n, S.obs, SC(n) and SC(2n): definitions are the same as in the TD output.

  • a1*,a2* = the number of singletons (a1*) and of doubletons (a2*) among the functionally indistinct set at the specified threshold level 'Tau'.

  • h1,h2 = the total contribution of singletons (h1) and of doubletons (h2) at the specified threshold level 'Tau'.

  • Tau = the specified threshold level of distinctiveness. Default is dmean (the mean distance between any two individuals randomly selected from the pooled data over all datasets).

## Data information for functional diversity (FDtype = 'AUC')
data(Brazil_rainforests)
data(Brazil_distM)
DataInfobeta3D(data = Brazil_rainforests, diversity = 'FD', datatype = 'abundance', 
               FDdistM = Brazil_distM, FDtype = 'AUC')
#>   Dataset        Assemblage   n S.obs SC(n) SC(2n) dmin dmean  dmax
#> 1   Marim              Edge 158    84 0.691  0.852    0 0.329 0.755
#> 2   Marim          Interior 144    80 0.704  0.899    0 0.313 0.663
#> 3   Marim Pooled assemblage 302   119 0.855  0.969    0 0.323 0.755
#> 4   Marim  Joint assemblage 302   164 0.696  0.876    0 0.323 0.755
#> 5  Rebio2              Edge 162    70 0.754  0.895    0 0.376 0.659
#> 6  Rebio2          Interior 168    74 0.763  0.877    0 0.310 0.660
#> 7  Rebio2 Pooled assemblage 330   118 0.819  0.901    0 0.355 0.770
#> 8  Rebio2  Joint assemblage 330   144 0.758  0.886    0 0.355 0.770

For FDtype = "AUC" the output information is described as follows:

  • Dataset, Pair, Assemblage, n, S.obs, SC(n) and SC(2n): definitions are the same as in TD and thus are omitted.

  • dmin = the minimum distance among all non-diagonal elements in the distance matrix.

  • dmean = the mean distance between any two individuals randomly selected from each assemblage.

  • dmax = the maximum distance among all elements in the distance matrix.

Below we use the demo dataset (Second-growth forests) to show the output of the function DataInfobeta3D for incidence data:

## Data information for taxonomic diversity with incidence data
data(Second_growth_forests)

DataInfobeta3D(data = Second_growth_forests, diversity = 'TD', datatype = 'incidence_raw',
               by_pair = FALSE)
#>              Dataset        Assemblage   T    U S.obs SC(T) SC(2T)  Q1 Q2 Q3 Q4 Q5
#> 1  CR_2005_2011_2017         Year_2005 100  787   135 0.919  0.953  64 17 16  6  4
#> 2  CR_2005_2011_2017         Year_2011 100  768   135 0.916  0.952  65 18 12  7  5
#> 3  CR_2005_2011_2017         Year_2017 100  768   134 0.917  0.956  64 20 11  8  3
#> 4  CR_2005_2011_2017 Pooled assemblage 100  929   152 0.926  0.961  69 22 15  6  6
#> 5  CR_2005_2011_2017  Joint assemblage 100 2323   404 0.917  0.953 193 55 39 21 12
#> 6  JE_2005_2011_2017         Year_2005 100  503    71 0.955  0.979  23  9  8  4  0
#> 7  JE_2005_2011_2017         Year_2011 100  631    88 0.942  0.962  37  8  4  6  7
#> 8  JE_2005_2011_2017         Year_2017 100  659    91 0.953  0.979  31 12  8  3  5
#> 9  JE_2005_2011_2017 Pooled assemblage 100  908   108 0.964  0.985  33 14  9  6  8
#> 10 JE_2005_2011_2017  Joint assemblage 100 1793   250 0.950  0.973  91 29 20 13 12

Information description:

  • Dataset = the input datasets.

  • Pair = combinations of assemblage pairs; this column is included only when the analysis is performed for every pair of assemblages.

  • Assemblage = Individual assemblages, 'Pooled assemblage' (for gamma) or 'Joint assemblage' (for alpha).

  • T = number of sampling units in the reference sample (sample size for incidence data).

  • U = total number of incidences in the reference sample.

  • S.obs = number of observed species in the reference sample.

  • SC(T) = sample coverage estimate of the reference sample.

  • SC(2T) = sample coverage estimate of twice the reference sample size.

  • Q1-Q5 = the first five species incidence frequency counts in the reference sample.

## Data information for taxonomic diversity for every pair of assemblages (incidence data)
data(Second_growth_forests)


DataInfobeta3D(data = Second_growth_forests, diversity = 'TD', datatype = 'incidence_raw', 
               by_pair = TRUE)
#>              Dataset                    Pair        Assemblage   T    U S.obs SC(T) SC(2T)  Q1 Q2 Q3 Q4
#> 1  CR_2005_2011_2017                                 Year_2005 100  787   135 0.919  0.953  64 17 16  6
#> 2  CR_2005_2011_2017                                 Year_2011 100  768   135 0.916  0.952  65 18 12  7
#> 3  CR_2005_2011_2017                                 Year_2017 100  768   134 0.917  0.956  64 20 11  8
#> 4  CR_2005_2011_2017 Year_2005 vs. Year_2011 Pooled assemblage 100  860   145 0.920  0.954  69 19 13  5
#> 5  CR_2005_2011_2017 Year_2005 vs. Year_2011  Joint assemblage 100 1555   270 0.917  0.952 129 35 28 13
#> 6  CR_2005_2011_2017 Year_2005 vs. Year_2017 Pooled assemblage 100  923   151 0.925  0.959  70 21 14  6
#> 7  CR_2005_2011_2017 Year_2005 vs. Year_2017  Joint assemblage 100 1555   269 0.918  0.954 128 37 27 14
#> 8  CR_2005_2011_2017 Year_2011 vs. Year_2017 Pooled assemblage 100  837   142 0.923  0.958  65 20 15  7
#> 9  CR_2005_2011_2017 Year_2011 vs. Year_2017  Joint assemblage 100 1536   269 0.917  0.954 129 38 23 15
#> 10 JE_2005_2011_2017                                 Year_2005 100  503    71 0.955  0.979  23  9  8  4
#> 11 JE_2005_2011_2017                                 Year_2011 100  631    88 0.942  0.962  37  8  4  6
#> 12 JE_2005_2011_2017                                 Year_2017 100  659    91 0.953  0.979  31 12  8  3
#> 13 JE_2005_2011_2017 Year_2005 vs. Year_2011 Pooled assemblage 100  757    96 0.951  0.969  37  8  6  7
#> 14 JE_2005_2011_2017 Year_2005 vs. Year_2011  Joint assemblage 100 1134   159 0.947  0.970  60 17 12 10
#> 15 JE_2005_2011_2017 Year_2005 vs. Year_2017 Pooled assemblage 100  864   107 0.963  0.987  32 17  9  4
#> 16 JE_2005_2011_2017 Year_2005 vs. Year_2017  Joint assemblage 100 1162   162 0.954  0.979  54 21 16  7
#> 17 JE_2005_2011_2017 Year_2011 vs. Year_2017 Pooled assemblage 100  788   100 0.958  0.981  33 13  8  6
#> 18 JE_2005_2011_2017 Year_2011 vs. Year_2017  Joint assemblage 100 1290   179 0.948  0.971  68 20 12  9

Output description: definitions are the same as before and thus are omitted.

License and feedback

The iNEXT.beta3D package is licensed under the GPLv3. To help refine iNEXT.beta3D, users’ comments or feedback would be welcome (please send them to Anne Chao or report an issue on the iNEXT.beta3D GitHub iNEXT.beta3D_github).

References

  • Chao, A., Chiu, C.-H., Hu, K.-H., and Zeleny, D. (2023a). Revisiting Alwyn H. Gentry’s forest transect data: a statistical sampling-model-based approach. Japanese Journal of Statistics and Data Science, 6, 861-884. (https://doi.org/10.1007/s42081-023-00214-1)

  • Chao, A., Henderson, P. A., Chiu, C.-H., Moyes, F., Hu, K.-H., Dornelas, M. and Magurran, A. E. (2021). Measuring temporal change in alpha diversity: a framework integrating taxonomic, phylogenetic and functional diversity and the iNEXT.3D standardization. Methods in Ecology and Evolution, 12, 1926-1940.

  • Chao, A., Thorn, S., Chiu, C.-H., Moyes, F., Hu, K.-H., Chazdon, R. L., Wu, J., Magnago, L. F. S., Dornelas, M., Zeleny, D., Colwell, R. K., and Magurran, A. E. (2023b). Rarefaction and extrapolation with beta diversity under a framework of Hill numbers: the iNEXT.beta3D standardization. Ecological Monographs e1588.